The diagnosis of a misfire event and the isolation of the cylinder in which the misfire took place is enforced by the onboard diagnostics (OBD) requirements over the whole operating range for all the vehicles, whatever the configuration of the engine they mount. This task is particularly challenging for engines with a high number of cylinders and for engine operating conditions that are characterized by high engine speed and low load. This is why much research has been devoted to this topic in recent years, developing different detection methodologies based on signals such as instantaneous engine speed, exhaust pressure, etc., both in time and frequency domains. This paper presents the development and the validation of a methodology for misfire detection based on the time-frequency analysis of the instantaneous engine speed signal. This signal contains information related to the misfire event, since a misfire occurrence is characterized by a sudden engine speed decrease and a subsequent damped torsional vibration. The identification of a specific pattern in the instantaneous engine speed frequency content, characteristic of the system under study, allows performing the desired misfire detection and cylinder isolation. Particular attention has been devoted to designing the methodology in order to avoid the possibility of false alarms caused by the excitation of this frequency pattern independently from a misfire occurrence. Although the time-frequency analysis is usually considered a time-consuming operation and not associated to onboard application, the methodology proposed here has been properly modified and simplified in order to obtain the quickness required for its use directly onboard a vehicle. Experimental tests have been performed on a $5.7l$ V12 spark-ignited engine run onboard a vehicle. The frequency characteristic of the engine-vehicle system is not the same that could be observed when running the engine on a test bench, because of the different inertia and stiffness that the connection between the engine and the load presents in the two cases. This makes it impossible to test and validate the methodology proposed here only on a test bench, without running tests on the vehicle. Nevertheless, the knowledge of the mechanical design of the engine and driveline gives the possibility of determining the resonance frequencies of the system (the lowest one is always the most important for this work) before running tests on the vehicle. This allows saving time and reducing costs in developing the proposed approach.

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