Crimp morphology is believed to be related to tendon mechanical behavior. While crimp has been extensively studied at slack or nondescript load conditions in tendon, few studies have examined crimp at specific, quantifiable loading conditions. Additionally, the effect of the number of cycles of preconditioning on collagen fiber crimp behavior has not been examined. Further, the dependence of collagen fiber crimp behavior on location and developmental age has not been examined in the supraspinatus tendon. Local collagen fiber crimp frequency is quantified throughout tensile mechanical testing using a flash freezing method immediately following the designated loading protocol. Samples are analyzed quantitatively using custom software and semi-quantitatively using a previously established method to validate the quantitative software. Local collagen fiber crimp frequency values are compared throughout the mechanical test to determine where collagen fiber frequency changed. Additionally, the effect of the number of preconditioning cycles is examined compared to the preload and toe-region frequencies to determine if increasing the number of preconditioning cycles affects crimp behavior. Changes in crimp frequency with age and location are also examined. Decreases in collagen fiber crimp frequency were found at the toe-region at all ages. Significant differences in collagen fiber crimp frequency were found between the preload and after preconditioning points at 28 days. No changes in collagen fiber crimp frequency were found between locations or between 10 and 28 days old. Local collagen fiber crimp frequency throughout mechanical testing in a postnatal developmental mouse SST model was measured. Results confirmed that the uncrimping of collagen fibers occurs primarily in the toe-region and may contribute to the tendon’s nonlinear behavior. Additionally, results identified changes in collagen fiber crimp frequency with an increasing number of preconditioning cycles at 28 days, which may have implications on the measurement of mechanical properties and identifying a proper reference configuration.
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April 2012
Research Papers
Examining Differences in Local Collagen Fiber Crimp Frequency Throughout Mechanical Testing in a Developmental Mouse Supraspinatus Tendon Model
Kristin S. Miller,
Kristin S. Miller
McKay Orthopaedic Research Laboratory,
University of Pennsylvania
, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia, PA 19104-6081
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Brianne K. Connizzo,
Brianne K. Connizzo
McKay Orthopaedic Research Laboratory,
University of Pennsylvania
, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia, PA 19104-6081
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Elizabeth Feeney,
Elizabeth Feeney
McKay Orthopaedic Research Laboratory,
University of Pennsylvania
, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia, PA 19104-6081
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Jennica J. Tucker,
Jennica J. Tucker
McKay Orthopaedic Research Laboratory,
University of Pennsylvania
, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia, PA 19104-6081
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Louis J. Soslowsky
Louis J. Soslowsky
McKay Orthopaedic Research Laboratory,
University of Pennsylvania
, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia, PA 19104-6081
Search for other works by this author on:
Kristin S. Miller
McKay Orthopaedic Research Laboratory,
University of Pennsylvania
, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia, PA 19104-6081
Brianne K. Connizzo
McKay Orthopaedic Research Laboratory,
University of Pennsylvania
, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia, PA 19104-6081
Elizabeth Feeney
McKay Orthopaedic Research Laboratory,
University of Pennsylvania
, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia, PA 19104-6081
Jennica J. Tucker
McKay Orthopaedic Research Laboratory,
University of Pennsylvania
, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia, PA 19104-6081
Louis J. Soslowsky
McKay Orthopaedic Research Laboratory,
University of Pennsylvania
, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia, PA 19104-6081J Biomech Eng. Apr 2012, 134(4): 041004 (7 pages)
Published Online: April 20, 2012
Article history
Received:
March 14, 2012
Revised:
March 15, 2012
Posted:
April 5, 2012
Published:
April 18, 2012
Online:
April 20, 2012
Citation
Miller, K. S., Connizzo, B. K., Feeney, E., Tucker, J. J., and Soslowsky, L. J. (April 20, 2012). "Examining Differences in Local Collagen Fiber Crimp Frequency Throughout Mechanical Testing in a Developmental Mouse Supraspinatus Tendon Model." ASME. J Biomech Eng. April 2012; 134(4): 041004. https://doi.org/10.1115/1.4006538
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