We describe an all-electronic, label-free, resistive pulse sensor that utilizes multiple micropores for simultaneous detection and counting of multiple biological particles. Four particle samples were utilized for the sensor testing: 20μm and 40μm polymethacrylate (PM) particles, Juniper pollen and Eastern Cottonwood pollen. Experiments demonstrated that this sensor was able to differentiate and count multiple particle solutions simultaneously through its four micropores fabricated on polymer membranes. Thus the sensing throughput has been improved significantly. Furthermore, the experimental results also proved the feasibility of differentiating various pollens from PM microparticles with the multi-pore resistive pulse sensor, with no need for labeling of samples.

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